One Step Quantitative Polymerase Chain Reaction Detection Kit

Classification: Detection Kit
Type: Detection Kit
Certification: CE, ISO13485
Group: All
Transport Package: Standard Packaging
Trademark: Refine Med
Customization:
Gold Member Since 2012

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Basic Info.

Model NO.
RF
Origin
Jiangsu, China
Production Capacity
10000000 Units/Year

Product Description

 
One Step Quantitative Polymerase Chain Reaction Detection Kit

[Detection Principle]
This kit adopts PCR method combined with fluorescence probe in vitro amplification technology. In this method, the HBV probe contains a fluorescent reporter dye FAM at the 5'end of the probe and a quencher dye BHQ at the 3'end of the probe. When the probe is intact, the proximity of the reporter dye to the quencher dye suppresses the reporter fluorescence. Probe cleavage during the PCR reaction spatially separates the reporter dye from the quencher dye, thereby allowing detection of the reporter dye fluorescence. The fragments of reporter dye are displaced from the target, resulting in an increase in fluorescence. This step, which enables the fluorescence signal accumulation and PCR products formed synchronous, thus to achieve qualitative detection the HBV in the infective patient in serum samples, which provide auxiliarymeans for the HBV infection in the treatment of the patient.
 
[Kit Contents]


Ref.
Type of reagentpresentationquantity
1HBV-pcr Rection Mix1 vial,1500μL1
2HBV Positive Control1 vial,60μL1
3Sample Dilution Buffer1 vial,1000μL1
4HBV Negative Control1 vial,200μL1
5User Manual11


HBV-PCR Rection Mix: Specific premiers and fluorescent probe of HBV, qRT-PCR Master mix.
HBV Positive Control: Pseudovirus with HBV NP gene established in vitro, Internal recombinant plasmid
Sample Dilution Buffer: PBS buffer
Note: Different batches in the kit components should not be interchangeable.
Analysis sensitivity: 20 copies/rxn.



About us

Refine Medical Technology Co., Ltd. was founded in Nanjing, China with the goal to supply medical products with high quality, competitive price and excellent after-sales service.
 
As the leading professional manufacturer & exporter of medical equipment, Refine Medical own a group of very professional technical and sales staff with the high efficiency, who are able to provide the customers complete range of service. Being approved with CE,ISO, Certificate, etc. We have established efficient worldwide network of marketing. In the past 15 years, we have exported our products to more than 120 countries all over the world. 
 
Refine Medical product range covers X-ray machines, Ultrasound Scanner, ECG, ICU Equipment, Laboratory Equipment, Optoelectronic Equipment, Reagents, Hospital Furniture and other medical equipments & products. 
 
We welcome customers from all over the world visit our website or our company, and we assure you of our best quality products and services with competitive prices.Your cooperation with us will definitely create a win-win situation for both of us.

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